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重組人心肌肌鈣蛋白T(cTnT)_8-TTC-rh_0.1mg

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一般類別:

    抗原?心臟標(biāo)志物?肌鈣蛋白T.

疾病類別:

    心臟標(biāo)志物?抗原?肌鈣蛋白T
同義詞名稱:
人心肌肌鈣蛋白T成人同種型,人心肌肌鈣蛋白T同種型#6或TnT3
資源:
在大腸桿菌中表達(dá)
蛋白質(zhì)序列:
基因TNNT2的產(chǎn)物;?288 AAR?
MSDIEEVVEE YEEEEQEEAA VEEQEEAAEE DAEAEAETEE TRAEEDEEEE EAKEAEDGPM EESKPKPRSF MPNLVPPKIP DGERVDFDDI HRKRMEKDLN ELQALIEAHF ENRKKEEEEL VSLKDRIERR RAERAEQQRI RNEREKERQN RLAEERARRE EEENRRKAED EARKKKALSNММHFGGYIQKQAQTERKSGK RQTEREKKKK ILAERRKVLA IDHLNEDQLR EKAKELWQSI YNLEAEKFDL QEKFKQQKYE INVLRNRIND NQKVSKTRGK AKVTGRWK?
重組人心肌肌鈣蛋白T包含在N-末端附加的蛋氨酸殘基(與天然蛋白比較在心肌細(xì)胞中表達(dá))。
應(yīng)用:
免疫測(cè)定中的校準(zhǔn)品或標(biāo)準(zhǔn)品,抗血清生產(chǎn)的免疫原,質(zhì)量cTnT標(biāo)準(zhǔn)品
分析:
純度> 95%(SDS-PAGE)。
通過分光光度法測(cè)定濃度,abs 0.1%(1g / L)-0.490(在280nm處測(cè)量)
免疫活動(dòng):
通過與幾種對(duì)人心臟TnT同種型的不同表位特異的單克隆抗體反應(yīng)來(lái)確認(rèn)免疫反應(yīng)性
純化:
免疫親和純化,然后進(jìn)行離子交換層析
介紹:
從0.01M HCl凍干
重建:
用Tris /尿素緩沖液(20mM Tris pH 7.5,7M尿素,5mM EDTA,15mMβ-巰基乙醇)重建。
儲(chǔ)存條件:
凍干-20°C(允許-15至-30°C)
重組-70°C(允許在-65至-80°C之間)
其他信息:
避免反復(fù)凍融。建議在重構(gòu)后將產(chǎn)品等分。
材料安全說(shuō)明:
該產(chǎn)品僅供研究使用。處理此材料時(shí)應(yīng)遵循標(biāo)準(zhǔn)實(shí)驗(yàn)室規(guī)范。

Recombinant human cardiac troponin T (cTnT)

Product Summary Synonym names:
Human cardiac troponin T adult isoform, human cardiac troponin T isoform #6 or TnT3 Source:
Expressed in E. coli Protein sequence:
Product of gene TNNT2; 288 a.a.r.
MSDIEEVVEE YEEEEQEEAA VEEQEEAAEE DAEAEAETEE TRAEEDEEEE EAKEAEDGPM EESKPKPRSF MPNLVPPKIP DGERVDFDDI HRKRMEKDLN ELQALIEAHF ENRKKEEEEL VSLKDRIERR RAERAEQQRI RNEREKERQN RLAEERARRE EEENRRKAED EARKKKALSN ММHFGGYIQK QAQTERKSGK RQTEREKKKK ILAERRKVLA IDHLNEDQLR EKAKELWQSI YNLEAEKFDL QEKFKQQKYE INVLRNRIND NQKVSKTRGK?AKVTGRWK Recombinant human cardiac TnT contains additional methionine residue at the N-terminus (in comparison with native protein expressed in cardiomyocytes).
Applications:
Calibrator or standard in immunoassays, immunogen for antisera production, mass cTnT standard Analysis:
Purity > 95 % (SDS-PAGE).
Concentration is determined spectrophotometrically, abs 0.1% (1 g/L) - 0.490 (measured at 280 nm)
Immunological activity:
Immunoreactivity is confirmed by reaction with several monoclonal antibodies specific to different epitopes of human cardiac TnT isoform Purification:
Immunoaffinity purification followed by ion-exchange chromatography Presentation:
Lyophilized from 0.01 M HCl Reconstitution:
Reconstitute with Tris/urea buffer (20 mM Tris pH 7.5, 7 M urea, 5 mM EDTA, 15 mM β-mercaptoethanol.
Storage Conditions:
Lyophilized -20 °C (between -15 and -30 °C allowed)
Reconstituted -70 °C (between -65 and -80 °C allowed)
Other information:
Avoid repeated freezing and thawing. It is recommended to aliquot the product after reconstitution.
Material Safety Note:
This product is sold for research use only. Standard Laboratory Practices should be followed when handling this material.
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