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重組人心肌肌鈣蛋白T(cTnT)_8-TTC-rh_1mg

6,645.00?¥ 6645.0 CNY

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一般類別:

    抗原?心臟標志物?肌鈣蛋白T.

疾病類別:

    心臟標志物?抗原?肌鈣蛋白T
同義詞名稱:
人心肌肌鈣蛋白T成人同種型,人心肌肌鈣蛋白T同種型#6或TnT3
資源:
在大腸桿菌中表達
蛋白質序列:
基因TNNT2的產物;?288 AAR?
MSDIEEVVEE YEEEEQEEAA VEEQEEAAEE DAEAEAETEE TRAEEDEEEE EAKEAEDGPM EESKPKPRSF MPNLVPPKIP DGERVDFDDI HRKRMEKDLN ELQALIEAHF ENRKKEEEEL VSLKDRIERR RAERAEQQRI RNEREKERQN RLAEERARRE EEENRRKAED EARKKKALSNММHFGGYIQKQAQTERKSGK RQTEREKKKK ILAERRKVLA IDHLNEDQLR EKAKELWQSI YNLEAEKFDL QEKFKQQKYE INVLRNRIND NQKVSKTRGK AKVTGRWK?
重組人心肌肌鈣蛋白T包含在N-末端附加的蛋氨酸殘基(與天然蛋白比較在心肌細胞中表達)。
應用:
免疫測定中的校準品或標準品,抗血清生產的免疫原,質量cTnT標準品
分析:
純度> 95%(SDS-PAGE)。
通過分光光度法測定濃度,abs 0.1%(1g / L)-0.490(在280nm處測量)
免疫活動:
通過與幾種對人心臟TnT同種型的不同表位特異的單克隆抗體反應來確認免疫反應性
純化:
免疫親和純化,然后進行離子交換層析
介紹:
從0.01M HCl凍干
重建:
用Tris /尿素緩沖液(20mM Tris pH 7.5,7M尿素,5mM EDTA,15mMβ-巰基乙醇)重建。
儲存條件:
凍干-20°C(允許-15至-30°C)
重組-70°C(允許在-65至-80°C之間)
其他信息:
避免反復凍融。建議在重構后將產品等分。
材料安全說明:
該產品僅供研究使用。處理此材料時應遵循標準實驗室規范。

Recombinant human cardiac troponin T (cTnT)

Product Summary Synonym names:
Human cardiac troponin T adult isoform, human cardiac troponin T isoform #6 or TnT3 Source:
Expressed in E. coli Protein sequence:
Product of gene TNNT2; 288 a.a.r.
MSDIEEVVEE YEEEEQEEAA VEEQEEAAEE DAEAEAETEE TRAEEDEEEE EAKEAEDGPM EESKPKPRSF MPNLVPPKIP DGERVDFDDI HRKRMEKDLN ELQALIEAHF ENRKKEEEEL VSLKDRIERR RAERAEQQRI RNEREKERQN RLAEERARRE EEENRRKAED EARKKKALSN ММHFGGYIQK QAQTERKSGK RQTEREKKKK ILAERRKVLA IDHLNEDQLR EKAKELWQSI YNLEAEKFDL QEKFKQQKYE INVLRNRIND NQKVSKTRGK?AKVTGRWK Recombinant human cardiac TnT contains additional methionine residue at the N-terminus (in comparison with native protein expressed in cardiomyocytes).
Applications:
Calibrator or standard in immunoassays, immunogen for antisera production, mass cTnT standard Analysis:
Purity > 95 % (SDS-PAGE).
Concentration is determined spectrophotometrically, abs 0.1% (1 g/L) - 0.490 (measured at 280 nm)
Immunological activity:
Immunoreactivity is confirmed by reaction with several monoclonal antibodies specific to different epitopes of human cardiac TnT isoform Purification:
Immunoaffinity purification followed by ion-exchange chromatography Presentation:
Lyophilized from 0.01 M HCl Reconstitution:
Reconstitute with Tris/urea buffer (20 mM Tris pH 7.5, 7 M urea, 5 mM EDTA, 15 mM β-mercaptoethanol.
Storage Conditions:
Lyophilized -20 °C (between -15 and -30 °C allowed)
Reconstituted -70 °C (between -65 and -80 °C allowed)
Other information:
Avoid repeated freezing and thawing. It is recommended to aliquot the product after reconstitution.
Material Safety Note:
This product is sold for research use only. Standard Laboratory Practices should be followed when handling this material.
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